福建师范大学激光与光电子技术研究所, 福建省光子技术重点实验室 医学光电科学与技术教育部重点实验室, 福建 福州 350007
实验研究了偏振与非偏振激光对亚心形扁藻的促长作用、叶绿素含量以及色素粗提物吸收光谱等生物学效应的影响。采用488 nm,70 mW输出功率的氩激光,辐照亚心形扁藻,隔天取样进行细胞计数,培养至第6天提取叶绿素,测量叶绿素含量并扫描叶绿素的吸收光谱。实验结果表明,偏振激光与非偏振激光辐照对藻细胞密度、生长速率的促长作用区别不显著; 偏振性与否对叶绿素含量的累积没有明显的区别; 激光偏振性对色素粗提物的吸收光谱没有影响。激光偏振性与否均可对亚心形扁藻的生物效应产生有效的作用,激光偏振性与激光生物效应关系的研究有待于进一步深入。
生物光子学 激光生物效应 激光偏振性 亚心形扁藻
Author Affiliations
Abstract
Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education of China, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007
The Raman spectra from leukemic cell line (HL60) and normal human peripheral blood mononuclear cells (PBMCs) are obtained by confocal micro-Raman spectroscopy using near-infrared laser (785 nm) excitation. The scanning range is from 500 to 2000 cm^{-1}. The two average Raman spectra of normal PBMCs and carcinoma cells have clear differences because their structure and amount of nucleic acid, protein, and other major molecules are changed. The spectra are also compared and analyzed by principal component analysis (PCA) to demonstrate the two distinct clusters of normal and transformed cells. The sensitivity of this technique for identifying transformed cells is 100%.
300.6450 Spectroscopy, Raman 170.0170 Medical optics and biotechnology 170.1530 Cell analysis 170.1790 Confocal microscopy Chinese Optics Letters
2008, 6(12): 938
福建师范大学 医学光电教育部重点实验室, 福建 福州 350007
研究正常生理条件蛋白酶的活化情况仍然存在很多困难,将蛋白酶荧光探针技术和基于各种光学平台的显微成像技术有机地结合起来,可以最大限度地记录活细胞生理条件下蛋白酶活性变化的时空信息。综述了蛋白酶荧光探针技术及其与该类探针应用相关的新型显微成像技术在生物医学光子学领域的应用进展。
医用光学 生物医学光子学 蛋白酶荧光探针 显微成像 活细胞